Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.Application
Antibodies tested include p-Cofilin 1 (F-11): (A,B) and Cofilin (FL-166): (C,D).Application
Antibodies tested include p-Cofilin 1 (F-11): (A,B,C) and Cofilin (FL-166): (D,E,F).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Antibodies tested include p-Cofilin 1 (E-5): (A,B) and Cofilin (FL-166): (C,D).Application
Antibodies tested include p-Cofilin 1 (E-5): (A,B,C) and Cofilin (FL-166): (D,E,F).Application
Antibodies tested include p-Cofilin 1 (hSer 3)-R: (A,B) and Cofilin (FL-166): (C,D).Application
Antibodies tested include p-Cofilin 1 (hSer 3)-R: (A,B,C) and Cofilin (FL-166): (D,E,F).Application
Western blot analysis of Cofilin 1 expression in non-transfected 293T: (A), human Cofilin 1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Cofilin 1 expression in non-transfected 293T: (A), human Cofilin 1 transfected 293T: (B), Jurkat (C) and K-562 (D) whole cell lysates.Application
Western blot analysis of Cofilin 2 expression in non-transfected: (A) and mouse Cofilin 2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cofilin expression in non-transfected 293T: (A), human Cofilin 1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | Cofilin Antibody (FL-166) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cofilin is ubiquitously expressed in eukaryotic cells where it binds to actin, thereby regulating the rapid cycling of actin assembly and disassembly essential for cellular viability (1-4). Cofilin is a low molecular weight protein that binds to filamentous (F) actin by bridging two longitudinally associated actin subunits changing the F-actin filament twist (4). This process is allowed by the dephosphorylation of cofilin ser-3 by factors such as opsonized zymosan (5, 6). Lim kinase 1, a serine kinase, phosphorylates cofilin and renders it unable to bind and depolymerise F-actin (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cofilin 1, Cofilin 2, and to a lesser extent, ADF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-166 representing full length Cofilin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-166 representing full length Cofilin 1 of human origin |
| Entrez Gene | 1072 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Antibodies tested include p-Cofilin 1 (F-11): (A,B) and Cofilin (FL-166): (C,D).
Application
Antibodies tested include p-Cofilin 1 (F-11): (A,B,C) and Cofilin (FL-166): (D,E,F).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Antibodies tested include p-Cofilin 1 (E-5): (A,B) and Cofilin (FL-166): (C,D).
Application
Antibodies tested include p-Cofilin 1 (E-5): (A,B,C) and Cofilin (FL-166): (D,E,F).
Application
Antibodies tested include p-Cofilin 1 (hSer 3)-R: (A,B) and Cofilin (FL-166): (C,D).
Application
Antibodies tested include p-Cofilin 1 (hSer 3)-R: (A,B,C) and Cofilin (FL-166): (D,E,F).
Application
Western blot analysis of Cofilin 1 expression in non-transfected 293T: (A), human Cofilin 1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Cofilin 1 expression in non-transfected 293T: (A), human Cofilin 1 transfected 293T: (B), Jurkat (C) and K-562 (D) whole cell lysates.
Application
Western blot analysis of Cofilin 2 expression in non-transfected: (A) and mouse Cofilin 2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cofilin expression in non-transfected 293T: (A), human Cofilin 1 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Cofilin 1, Cofilin 2, and to a lesser extent, ADF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
