Application
Western blot analysis of Pinch-1/2 expression in T98G (A) and COLO 320DM (B) whole cell lysates.Application
Western blot analysis of Pinch-1/2 expression in human platelets whole cell lysate.| Product Name | Pinch-1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Pinch is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor and integrin signaling pathway. Pinch is involved in cell differentiation, proliferation and survival by acting as an effector of integrin and growth factor signaling. It is a cytoplasmic protein expressed in most tissues and consists of five LIM domains, a nuclear localization signal and a nuclear export signal. The PINCH-1/ILK complex is regulated by another member of the Pinch family, PINCH-2, which also forms a complex with ILK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Pinch-1 and Pinch-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Pinch-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Pinch-1 of human origin |
| Entrez Gene | 3987 |
|---|
Application
Western blot analysis of Pinch-1/2 expression in T98G (A) and COLO 320DM (B) whole cell lysates.
Application
Western blot analysis of Pinch-1/2 expression in human platelets whole cell lysate.| Application Notes | recommended for detection of Pinch-1 and Pinch-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
