Application
Western blot analysis of ULK1 expression in non-transfected 293T: (A), human ULK1 transfected 293T: (B) and Jurkat (C) whole cell lysates.| Product Name | ULK1 Antibody (H-240) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | ULK1 and ULK2 (for UNC-51-like kinase) encode similar amino-terminal serine/threonine kinase domains, a proline/serine-rich (PS) domain, and a species conserved carboxyl-terminal domain (1,2). Both share homology with the UNC-51 kinase from Caenorhabditis elegans and the APG1 kinase in yeast, which are involved in axonal extension and growth, and autophagy, respectively (1-5). ULK1 maps to human chromosome 12q24.3 and is ubiquitously expressed (6). ULK2, also widely expressed, maps to mouse chromosome 11B1.3 and is expected to have a similar molecular weight as ULK1 in human (2). ULK1 and ULK2 are thought to auto-phosphorylate the PS domain in vitro, and the significant homology among vertebrates suggest that ULK1 and ULK2 are involved in the regulation of fundamental biological processes (1,2,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ULK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 511-750 mapping within an internal region of ULK1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 511-750 mapping within an internal region of ULK1 of human origin |
| Entrez Gene | 8408 |
|---|
Application
Western blot analysis of ULK1 expression in non-transfected 293T: (A), human ULK1 transfected 293T: (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of ULK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
