Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of NOR-1 expression in non-transfected: (A) and mouse NOR-1 transfected: (B) 293 whole cell lysates.| Product Name | NOR-1 Antibody (H-180) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Nur77 (also designated NGFI-B), Nurr1 (Nur-related factor 1), and NOR-1 (neuron-derived orphan receptor 1) constitute the NGFI-B subfamily within the nuclear receptor superfamily. Ligands for these protein have not been identified, and, therefore, they are designated “orphan nuclear receptors”. Genes of the NGFI-B subfamily are classified as immediate-early genes, which are induced rapidly, but transiently, in response to a variety of stimuli. They have been implicated in cell proliferation, differentiation, and apoptosis. The human NOR-1 gene maps to chromosome 9q, and encodes a protein which is expressed in heart, skeletal muscle, thymus, and spleen as well as in brain, where it is developmentally regulated. There-fore, NOR-1 may be involved in regulating neural differentiation. The NOR-1 gene also undergoes chromosomal translocation with the EWS gene to produce a protein thought to affect pre-mRNA splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of NOR-1 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 111-200 mapping within an internal region of NOR-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 111-200 mapping within an internal region of NOR-1 of human origin |
| Entrez Gene | 8013 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of NOR-1 expression in non-transfected: (A) and mouse NOR-1 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of NOR-1 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
