Application
Western blot analysis of CPEB expression in non-transfected: (A) and human CPEB transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CPEB expression in non-transfected: (A) and human CPEB transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CPEB expression in HeLa whole cell lysate.| Product Name | CPEB Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The regulated translation of messenger RNA is essential for cell-cycle progression, establishment of the body plan during early development, and modulation of key activities in the central nervous system (1). Cytoplasmic polyadenylation, one mechanism of controlling translation, is driven by cytoplasmic polyadenylation element binding protein, CPEB. CPEB is a highly conserved, sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element, thereby modulating translational repression and mRNA localization (1–5). Blocking cytoplasmic polyadenylation by interfering with the CPE or CPEB prevents the translational activation and translational repression of mRNAs crucial for oocyte maturation (2–5). CPEB is synthesized during oogenesis and stockpiled in the oocyte (2). CPEB degradation occurs via the proteasome pathway, most likely through ubiquitin-conjugated intermediates (2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of CPEB long and short isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of CPEB of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of CPEB of human origin |
| Entrez Gene | 64506 |
|---|
Application
Western blot analysis of CPEB expression in non-transfected: (A) and human CPEB transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CPEB expression in non-transfected: (A) and human CPEB transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CPEB expression in HeLa whole cell lysate.| Application Notes | recommended for detection of CPEB long and short isoforms of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
