Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of beta;-TrCP expression in non-transfected: (A) and mouse beta;-TrCP transfected: (B) 293T whole cell lysates.| Product Name | β-TrCP Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 26-110 mapping near the N-terminus of β-TrCP of human origin |
| Modification Notes | ß-TrCP (beta-tranducin repeats containing protein), also designated E3RSIkB or FWD1, and HOS (homologous to Slimb) are F-box proteins that function as substrate recognition subunits of ubiquitin ligases (1-5). HOS and ß-TrCP differ in their amino terminal regions, but exhibit high homology within the F-box and WD40 repeat-containing regions (3). ß-TrCP mediates ubiquitin/proteasome-dependent degradation of CD4 and ubiquitination of various proteins including IkB and ß-catenin (1,5). HOS has also been shown to regulate the degradation of IkB and ß-catenin in a similar manner (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of β-TrCP isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 26-110 mapping near the N-terminus of &beta -TrCP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 26-110 mapping near the N-terminus of β-TrCP of human origin |
| Entrez Gene | 8945 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of beta;-TrCP expression in non-transfected: (A) and mouse beta;-TrCP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of β-TrCP isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
