Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of Nek2 expression in K-562 whole cell lysate.Application
Western blot analysis of Nek2 expression in K-562 whole cell lysate (A) and HeLa (B) and K-562 (C) nuclear extracts.| Product Name | Nek2 Antibody (H-235) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 211-445 mapping at the C-terminus of Nek2 of human origin |
| Modification Notes | NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis. NIMA-related mammalian proteins have since been identified as Nek1, Nek2, Nek3 and Nek4 (also designated STK2 or NRK2). High expression of Nek1 is seen in male and female germ cell lines of mouse. Nek2 is the closest known mammalian relative to NIMA. Like NIMA, Nek2 expression peaks at the G2 to M phase transition. Nek3 is a predominantly cytoplasmic enzyme that was detectable in all organs studied. Levels of Nek3 seem to remain unchanged throughout the cell cycle, but appear to be elevated in G0-arrested, quiescent fibroblasts. In developing testicular germ cells, differential patterns of expression were seen for Nek1, Nek2 and Nek4, indicating possible overlapping, but non-identical functions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Nek2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 211-445 mapping at the C-terminus of Nek2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 211-445 mapping at the C-terminus of Nek2 of human origin |
| Entrez Gene | 4751 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of Nek2 expression in K-562 whole cell lysate.
Application
Western blot analysis of Nek2 expression in K-562 whole cell lysate (A) and HeLa (B) and K-562 (C) nuclear extracts.| Application Notes | recommended for detection of Nek2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
