Application
Antibodies tested include p-MYPT1 (Ser 695): (A, B, C) and MYPT1 (H-130): (D, E, F).Application
Western blot analysis of phosphorylated MYPT1 expression in non-transfected: (A) and human MYPT1 transfected: (B) 293 whole cell lysates.| Product Name | p-MYPT1 Antibody (Ser 695) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Myosin phosphatase target subunit 1 (MYPT1), also called myosin-binding subunit of myosin phosphatase, is one of the subunits and an integral component of the myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, which inhibits myosin phosphatase through the action of Rho-kinase. MYPT1 promotor contains one Sp1 transcription factor binding site, suggesting that MYPT1 is a housekeeping gene. Myotonic dystrophy protein kinase phosphorylates MYPT1 at tyrosine 654 to regulate myosin II phosphorylation. Inhibition of myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated myosin light chain to MYPT1. PP1c interacts at least four binding sties on the amino-terminus of MYPT1. A novel isoform of MYPT1, MYPT2, also interacts with PPlc. MYPT1 is localized on stress fibers, and it is distributed close to the cell membrane and at cell-cell contacts to regulate myosin phosphatase activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 695 phosphorylated MYPT1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 695 of MYPT1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 695 of MYPT1 of human origin |
| Entrez Gene | 4659 |
|---|
Application
Antibodies tested include p-MYPT1 (Ser 695): (A, B, C) and MYPT1 (H-130): (D, E, F).
Application
Western blot analysis of phosphorylated MYPT1 expression in non-transfected: (A) and human MYPT1 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of Ser 695 phosphorylated MYPT1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
