Application
Western blot analysis of RORγ expression in non-transfected: (A) and human RORγ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RORγ expression in non-transfected: (A) and human RORγ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RORγ expression in rat skeletal muscle tissue extract.| Product Name | RORγ Antibody (H-190) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The nuclear orphan receptors RORå and ROR© are members of the nuclear hormone receptor superfamily. This family acts by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORå and ROR© are unique in that they bind DNA as monomers. RORå has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. ROR© comprises a 560 amino acid protein that shares 50% amino acid identity with RORå and is most highly expressed in skeletal muscle. Although these proteins are considered “orphan receptors,” due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors. The gene encoding RORå maps to chromosome 15q22.2 and the gene encoding ROR© maps to chromosome 1q21.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPELISAGSIFIPWB |
| recommended for detection of RORγ of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-320 mapping within an internal region of RORγ of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-320 mapping within an internal region of RORγ of human origin |
| Entrez Gene | 6097 |
|---|
Application
Western blot analysis of RORγ expression in non-transfected: (A) and human RORγ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RORγ expression in non-transfected: (A) and human RORγ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RORγ expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of RORγ of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
