Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells and Islets of Langerhans.Application
Western blot analysis of PDE5A expression in non-transfected: (A) and human PDE5A transfected: (B) 293 whole cell lysates.Application
Western blot analysis of PDE5A expression in non-transfected: (A) and human PDE5A transfected: (B) 293 whole cell lysates.| Product Name | PDE5A Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Phosphodiesterases (PDE, also designated cyclic nucleotide phosphodiesterase) are important for the downregulation of the intracellular level of the second messenger cyclic adenosine monophosphate (cAMP) by hydrolyzing cAMP to 5'AMP. The PDE family contains proteins that serve tissue-specific roles in the regulation of lipolysis, glycogenolysis, myocardial contractility and smooth muscle relaxation. PDE5A, also designated cGMP-binding cGMP-specific phosphodiesterase or CGB-PDE, regulates the intracellular concentration of cyclic nucleotides and thereby is important in signal transduction. PDE5A catalyzes the hydrolysis of cGMP to 5’GMP and the protein is expressed in heart, placenta, aortic smooth muscle cells, skeletal muscle and pancreas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PDE5A isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-150 mapping near the N-terminus of PDE5A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-150 mapping near the N-terminus of PDE5A of human origin |
| Entrez Gene | 8654 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells and Islets of Langerhans.
Application
Western blot analysis of PDE5A expression in non-transfected: (A) and human PDE5A transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of PDE5A expression in non-transfected: (A) and human PDE5A transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of PDE5A isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
