Application
Western blot analysis of BLNK expression in Ramos whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells and lymphoid tissue (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of BLNK expression in BJAB (A), Ramos (B) and HuT 78 (C) whole cell lysates.| Product Name | BLNK Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cross-linking of the B cell receptor (BCR) activates a variety of signaling pathways involved in processes such as cell proliferation and apoptosis. Intracellular protein tyrosine kinases such as Syk and Lyn have been implicated in this BCR signal transduction and are thought to play an important role in B cell development. BLNK (for B cell linker protein) is a central linker protein in B cells which has been shown to associate with the effector proteins GRB2, Vav, Nck and PLC © following activation of the B cell receptor. The two forms of BLNK, pp68 and pp70, arise from alternate splicing of the human BLNK gene transcript. BLNK is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLC ©. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BLNK p70 and BLNK p68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 201-280 mapping to an internal region of BLNK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 201-280 mapping to an internal region of BLNK of human origin |
| Entrez Gene | 29760 |
|---|
Application
Western blot analysis of BLNK expression in Ramos whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells and lymphoid tissue (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of BLNK expression in BJAB (A), Ramos (B) and HuT 78 (C) whole cell lysates.| Application Notes | recommended for detection of BLNK p70 and BLNK p68 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
