Application
Antibodies tested include p-RKIP (hSer 153): (A, B) and RKIP (FL-187): (C, D).| Product Name | p-RKIP Antibody (hSer 153) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Raf kinase inhibitory protein (RKIP, PEBP) is a modulator of the Raf/MAPK signaling cascade and a suppressor of metastatic cancer. RKIP inhibits MAPK by preventing association of Raf-1 and p21-activated kinase (PAK), and blocking phosphorylation of the Raf-1 kinase domain by PAK and Src kinases. After G protein receptor stimulation, RKIP can dissociate Raf-1 and associate with GRK2, thereby blocking GRK2 activity. This switch is triggered by protein kinase C (PKC)-dependent phosphorylation of the RKIP on Serine 153. RKIP Serine 153 phosphorylation by PKC in response to phorbol ester or epidermal growth factor causes release of RKIP from Raf-1. RKIP antagonizes the signal transduction pathways that mediate the activation of NFkB in response to stimulation with TNF-a or interleukin-1 b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| blocking peptide, sc-32623 P | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 153 phosphorylated RKIP of human origin by WB IP IF and ELISA also reactive with additional species including equine canine bovine and porcine |
|---|---|
| Tissue Specificity | recommended for detection of Ser 153 phosphorylated RKIP of human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine |
| Entrez Gene | 5037 |
|---|
Application
Antibodies tested include p-RKIP (hSer 153): (A, B) and RKIP (FL-187): (C, D).| Application Notes | blocking peptide, sc-32623 P |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
