Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic and membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of A cyclase VIII expression in IMR-32 whole cell lysate.| Product Name | A cyclase VIII Antibody (H-270) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Ga s) for GTP, dissociation of GTP-Gå s from G∫ © and Gå s-mediated activation of adenylyl cyclase. Adenylyl cyclase type VIII (A cyclase VIII) is one of the three mammalian calcium-stimulated isoforms, each of which is expressed in a region-specific manner in the central nervous system. In addition to the high expression in the brain, A cyclase VIII is also expressed in the lung. Ca2+/calmodulin-dependent A cyclase VIII immunoreactivity is increased in alcoholic corpus amyadaloideum and hippocampus, suggesting that adenyl cyclase may play a role in the pathophysiology of alcoholism. A significant decrease in the level of A cyclase I and a tendency to decrease in the level of A cyclase VIII in Alzheimer’s disease hippocampus suggests that A cyclase I and VIII may play an essential role in learning and memory. A cyclase VIII knock-out mice do not have normal increases in behavioral markers of anxiety; thus, A cyclase VIII may also function in the modulation of anxiety. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of A cyclase VIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 561-830 mapping within an internal region of A cyclase VIII of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 561-830 mapping within an internal region of A cyclase VIII of human origin |
| Entrez Gene | 114 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic and membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of A cyclase VIII expression in IMR-32 whole cell lysate.| Application Notes | recommended for detection of A cyclase VIII of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
