Application
Western blot analysis of Fibronectin expression in Hep G2 (A) and NCI-H929 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic and extracellular localization.Application
Western blot analysis of Fibronectin expression in Hep G2 whole cell lysate.| Product Name | Fibronectin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren’s disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Fibronectin of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2087-2386 mapping at the C-terminus of Fibronectin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2087-2386 mapping at the C-terminus of Fibronectin of human origin |
| Entrez Gene | 2335 |
|---|
Application
Western blot analysis of Fibronectin expression in Hep G2 (A) and NCI-H929 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic and extracellular localization.
Application
Western blot analysis of Fibronectin expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of Fibronectin of mouse, rat, human, zebrafish and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
