Application
Western blot analysis of B7-2 expression in non-transfected 293T: (A), human B7-2 transfected 293T: (B) and Raji (C) whole cell lysates.Application
Western blot analysis of B7-2 expression in non-transfected: (A) and human B7-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of B7-2 expression in Jurkat whole cell lysate.| Product Name | B7-2 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins B7-1 (CD80) and B7-2 (CD86) expressed on antigen presenting cells bind the homologous T cell receptors CD28 and CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28, and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of B7-2 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 24-223 of B7-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 24-223 of B7-2 of human origin |
| Entrez Gene | 942 |
|---|
Application
Western blot analysis of B7-2 expression in non-transfected 293T: (A), human B7-2 transfected 293T: (B) and Raji (C) whole cell lysates.
Application
Western blot analysis of B7-2 expression in non-transfected: (A) and human B7-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of B7-2 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of B7-2 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
