Application
Western blot analysis of Skip expression in non-transfected: (A) and mouse Skip transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of SKIP expression in non-transfected: (A) and mouse SKIP transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of Skip expression in HeLa (A) and DU 145 (B) nuclear extracts.| Product Name | Skip Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 237-536 mapping at the C-terminus of Skip of human origin |
| Modification Notes | Ski is a unique oncoprotein that is involved in inducing both transformation and differentiation. Skip (Ski-interacting protein) is a nuclear hormone receptor that binds the highly-conserved region of Ski, which is required for its transforming activity. Skip is involved in Vitamin D-mediated transcription. Specifically, Skip interacts with E7, the major transforming protein of human papillomavirus, which is implicated in the development of cervical cancer. Skip has specific inhibitory effects on BMP-2-induced differentiation and is implicated to be a novel regulator of the differentiation programming induced by TGF-beta signals. Skip also functions as a repressor in Notch signalling in association with the corepressor SMRT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Skip (Ski-Interacting protein) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 237-536 mapping at the C-terminus of Skip of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 237-536 mapping at the C-terminus of Skip of human origin |
| Entrez Gene | 22938 |
|---|
Application
Western blot analysis of Skip expression in non-transfected: (A) and mouse Skip transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of SKIP expression in non-transfected: (A) and mouse SKIP transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of Skip expression in HeLa (A) and DU 145 (B) nuclear extracts.| Application Notes | recommended for detection of Skip (Ski-Interacting protein) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
