Application
Western blot analysis of CD40 expression in non-transfected: (A) and mouse CD40 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD40 expression in NAMALWA whole cell lysate.Application
Western blot analysis of CD40 expression in non-transfected: (A) and human CD40 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of CD40 expression in non-transfected control (A) and CD40 siRNA transfected (B) BJAB cells. Blot probed with CD40 (C-20): . Actin (I-19): used as specificity and loading control.Application
Immunofluorescence staining of methanol-fixed BJAB cells showing membrane localization.Application
Western blot analysis of CD40 expression in BJAB (A), A-431 (B), NAMALWA (C) and Ramos (D) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing membrane staining.| Product Name | CD40 Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of CD40 of human origin |
| Modification Notes | Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. A receptor ligand pair central to the transmission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif. Other members of the family include TRAF1 and TRAF2. The latter proteins have been shown to regulate TNF-R2 as well as CD40 signaling through activation of the NFkB family of transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD40 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of CD40 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of CD40 of human origin |
| Entrez Gene | 958 |
|---|
Application
Western blot analysis of CD40 expression in non-transfected: (A) and mouse CD40 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD40 expression in NAMALWA whole cell lysate.
Application
Western blot analysis of CD40 expression in non-transfected: (A) and human CD40 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of CD40 expression in non-transfected control (A) and CD40 siRNA transfected (B) BJAB cells. Blot probed with CD40 (C-20): . Actin (I-19): used as specificity and loading control.
Application
Immunofluorescence staining of methanol-fixed BJAB cells showing membrane localization.
Application
Western blot analysis of CD40 expression in BJAB (A), A-431 (B), NAMALWA (C) and Ramos (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing membrane staining.| Application Notes | recommended for detection of CD40 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
