Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of Leukocyte-type 12-LO expression in non-transfected: (A) and mouse Leukocyte-type 12-LO transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 15-LO expression in non-transfected 293T: (A), human 15-LO transfected 293T: (B) and human PBL (C) whole cell lysates.| Product Name | 15-LO Antibody (H-235) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 428-662 mapping at the C-terminus of 15-LO of human origin |
| Modification Notes | Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes and synthesis of signaling molecules, as well as inducing structural and metabolic changes in the cell. The Lox enzymes in mammals include 12-LO and 15-LO, which are classified with respect to their positional specificity of the deoxygenation of their most common substrate, arachidonic acid. The metabolism of arachidonic acid leads to the generation of biologically active metabolites that have been implicated in cell growth and proliferation, as well as survival and apoptosis. 15-Lipoxygenase (15-LO) acts in physiological membrane remodeling and the pathogenesis of atherosclerosis, inflammation, and carcinogenesis. It is highly regulated and expressed in a tissue- and cell-type-specific fashion. IL-4 and IL-13 play important roles in transactivating the 15-LO gene. Overexpression of 15-LO type 1 in prostate cancer contributes to the cancer progression by regulating IGF-1R expression and activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of 15-lipoxygenase and leukocyte-, platelet- and epidermal-type 12-lipoxygenases of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 428-662 mapping at the C-terminus of 15-LO of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 428-662 mapping at the C-terminus of 15-LO of human origin |
| Entrez Gene | 246 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of Leukocyte-type 12-LO expression in non-transfected: (A) and mouse Leukocyte-type 12-LO transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 15-LO expression in non-transfected 293T: (A), human 15-LO transfected 293T: (B) and human PBL (C) whole cell lysates.| Application Notes | recommended for detection of 15-lipoxygenase and leukocyte-, platelet- and epidermal-type 12-lipoxygenases of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
