Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in glomeruli and tubules.Application
Western blot analysis of NELF-E expression in 293T whole cell lysate.| Product Name | NELF-E Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NELF-E, for negative elongation factor E, is a putative RNA binding protein (1). NELF-E is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation (1). Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF (2). DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites (3). DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT (4). NELF-E is also known as RD and RDBP (RD RNA-binding protein). (5,6). RD, the acronym of the most common dipeptide repeat describes the single letter symbols for arginine (R) and aspartic acid (D), respectively (5). NELF-E has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions (7). The human NELF-E gene maps to chromosome 6p21.3 and encodes a 371 amino acid protein (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NELF-E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 241-380 mapping at the C-terminus of NELF-E of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 241-380 mapping at the C-terminus of NELF-E of human origin |
| Entrez Gene | 7936 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear and cytoplasmic staining of cells in glomeruli and tubules.
Application
Western blot analysis of NELF-E expression in 293T whole cell lysate.| Application Notes | recommended for detection of NELF-E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
