Application
Western blot analysis of CD40 expression in non-transfected: (A) and human CD40 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD40 expression in non-transfected: (A) and mouse CD40 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD40 expression in NAMALWA whole cell lysate.Application
Western blot analysis of CD40 expression in non-transfected: (A) and human CD40 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of CD40 expression in BJAB whole cell lysate.Application
Western blot analysis of human recombinant CD40 fusion protein.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane localization.| Product Name | CD40 Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. A receptor ligand pair central to the transmission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif. Other members of the family include TRAF1 and TRAF2. The latter proteins have been shown to regulate TNF-R2 as well as CD40 signaling through activation of the NFkB family of transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD40 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 74-193 mapping within the extracellular domain of CD40 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 74-193 mapping within the extracellular domain of CD40 of human origin |
| Entrez Gene | 958 |
|---|
Application
Western blot analysis of CD40 expression in non-transfected: (A) and human CD40 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD40 expression in non-transfected: (A) and mouse CD40 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD40 expression in NAMALWA whole cell lysate.
Application
Western blot analysis of CD40 expression in non-transfected: (A) and human CD40 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of CD40 expression in BJAB whole cell lysate.
Application
Western blot analysis of human recombinant CD40 fusion protein.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane localization.| Application Notes | recommended for detection of CD40 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
