Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.Application
Western blot analysis of Rap 1B expression in non-transfected: (A) and mouse Rap 1B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rap 1B expression in non-transfected 293T: (A), mouse Rap 1B transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of Rap 1 expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Rap 1 expression in A-431 =1482(A), SW480 (B) and NIH/3T3 (C) whole cell lysates.| Product Name | Rap 1 Antibody (121) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ras oncogenes encode GTP-binding proteins that are capable of transforming immortalized cells in culture. Two Ras-related human genes, designated RAP1A and RAP1B, encode 95% homologous proteins that share a similar C-terminal Cys-Ali-Ali-Xaa sequence with Ras proteins and are ubiquitously expressed in mammalian tissues. The putative “effector” domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. It has been postulated that p21Rap1 acts to interfere with Ras effector function by binding to Ras GAP. In fact it is known that p21Rap1 binds to Ras GAP in vitro in a GTP dependent manner without affecting p21Rap1 GTPase activity. The Rap 2 protein shares 60% identity with the Rap 1A protein and exhibits a carboxy terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap 1, overexpression of Rap 2 does not interfere with the Ras signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rap 1A and Rap 1B p21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of Rap 1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of Rap 1 of human origin |
| Entrez Gene | 5906 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.
Application
Western blot analysis of Rap 1B expression in non-transfected: (A) and mouse Rap 1B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rap 1B expression in non-transfected 293T: (A), mouse Rap 1B transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of Rap 1 expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Rap 1 expression in A-431 =1482(A), SW480 (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of Rap 1A and Rap 1B p21 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
