Application
Western blot analysis of RECK expression in AT-3 whole cell lysate.| Product Name | RECK Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | RECK (reversion-inducing-cysteine-rich protein with Kazal motifs) is a membrane anchored glycoprotein that binds to and inhibits the proteolytic activity of matrix metalloproteinase-9 (MMP-9) (1). The enzymatic activity of MMP-9 facilitates tumor invasion by proteolytically digesting the extracellular matrix, thereby enabling tumor growth, expansion and metastasis (2). RECK inhibits the secretion and activation of MMP-9 into the extracellular matrix, which results in the inhibition of tumor growth (3). RECK contains multiple EGF-like repeats and serine-protease inhibitor-like domains (4). The expression of RECK is suppressed in several tumors and oncogenically transformed cells, suggesting that the loss of RECK activity correlates with transformed phenotypes (5,6). Transcriptional activation of RECK is potentially negatively regulated by the Sp1 family of transcription factors, as it contains two Sp1 binding motifs in the promoter region, and, in cells transformed with the ras oncogene, the Sp1 promoter region is essential for repressing RECK gene expression (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RECK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 644-943 mapping near the C-terminus of RECK of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 644-943 mapping near the C-terminus of RECK of human origin |
| Entrez Gene | 8434 |
|---|
Application
Western blot analysis of RECK expression in AT-3 whole cell lysate.| Application Notes | recommended for detection of RECK of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
