Application
Antibodies tested include p-Lck (pY505.4): (A,B,C) and Lck (H-95): (D,E,F).Application
Antibodies tested include p-Lck (Tyr 393): (A,B) and Lck (H-95): (C,D).Application
Antibodies tested include p-Lck (Tyr 192/Ser 194)-R: (A,B) and Lck (H-95): (C,D).Application
Antibodies tested include p-Lck (Tyr 192/Ser 194)-R: (A,B,C) and Lck (H-95): (D,E,F).Application
Antibodies tested include p-Lck (Tyr 393): (A,B,C) and Lck (H-95): (D,E,F).Application
Western blot analysis of Lck expression in non-transfected: (A) and human Lck transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Lck expression in non-transfected: (A) and human Lck transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Lck expression in non-transfected 293T: (A), human Lck transfected 293T: (B) and HuT 78 (C) whole cell lysates.Application
Western blot analysis of Lck expression in non-transfected 293T: (A), mouse Lck transfected 293T: (B) and HuT 78 (C) whole cell lysates.Application
Western blot analysis of Lck expression in CCRF HSB-2 (A), Jurkat (B) and MOLT-4 (C) whole cell lysates.| Product Name | Lck Antibody (H-95) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus, or ASV. Src was the first proto-oncogenic, non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src family kinases contain an amino terminal cell membrane anchor, followed by an SH3 domain and an SH2 domain that are involved in modular association and activation, respectively. Src family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src family kinases may be important for the regulation of specific cellular processes, such as mitogenesis, cytoskeletal organization and membrane trafficking. The Fyn and Lck Src family tyrosine kinases play a key role in T cell antigen receptor (TCR) signaling. The human Lck gene maps to chromosome 1p35.1 and encodes a 509 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Lck p56 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-95 mapping at the N-terminus of Lck of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-95 mapping at the N-terminus of Lck of human origin |
| Entrez Gene | 3932 |
|---|
Application
Antibodies tested include p-Lck (pY505.4): (A,B,C) and Lck (H-95): (D,E,F).
Application
Antibodies tested include p-Lck (Tyr 393): (A,B) and Lck (H-95): (C,D).
Application
Antibodies tested include p-Lck (Tyr 192/Ser 194)-R: (A,B) and Lck (H-95): (C,D).
Application
Antibodies tested include p-Lck (Tyr 192/Ser 194)-R: (A,B,C) and Lck (H-95): (D,E,F).
Application
Antibodies tested include p-Lck (Tyr 393): (A,B,C) and Lck (H-95): (D,E,F).
Application
Western blot analysis of Lck expression in non-transfected: (A) and human Lck transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Lck expression in non-transfected: (A) and human Lck transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Lck expression in non-transfected 293T: (A), human Lck transfected 293T: (B) and HuT 78 (C) whole cell lysates.
Application
Western blot analysis of Lck expression in non-transfected 293T: (A), mouse Lck transfected 293T: (B) and HuT 78 (C) whole cell lysates.
Application
Western blot analysis of Lck expression in CCRF HSB-2 (A), Jurkat (B) and MOLT-4 (C) whole cell lysates.| Application Notes | recommended for detection of Lck p56 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
