Application
Western blot analysis of eIF2β expression in KNRK whole cell lysate.Application
Western blot analysis of eIF2β expression in KNRK (A), HeLa (B), Jurkat (C), K-562 (D), A-431 (E) and NIH/3T3 (F) whole cell lysates.| Product Name | eIF2β Antibody (H-203) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2 exists as a heterotrimeric complex of eIF2å, eIF2ß and eIF2© (1-3). eIF2 functions in the early stages of protein synthesis, by forming a ternary complex with GTP and tRNA. This complex binds to the 40S ribosomal subunit, followed by mRNA binding to 40S to form the 43S preinitiation complex, the release of eIF2 from 40S, and the hydrolysis of GTP (4,5). Phosphorylation of eIF2a correlates with inhibition of translation initiation (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of eIF2&beta of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 131-333 mapping at the C-terminus of eIF2&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 131-333 mapping at the C-terminus of eIF2β of human origin |
| Entrez Gene | 8894 |
|---|
Application
Western blot analysis of eIF2β expression in KNRK whole cell lysate.
Application
Western blot analysis of eIF2β expression in KNRK (A), HeLa (B), Jurkat (C), K-562 (D), A-431 (E) and NIH/3T3 (F) whole cell lysates.| Application Notes | recommended for detection of eIF2&beta of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
