Application
Western blot analysis of TCP-1 β expression in non-transfected: (A) and mouse TCP-1 β transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in ductus seminiferus and Leydig cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of TCP-1 β expression in mouse testis tissue extract (A) and ZR-75-1 (B), F9 (C), HeLa (D) and HOS (E) whole cell lysates.Application
Western blot analysis of TCP-1 β expression in mouse testis tissue extract (A) and HeLa (B) and ZR-75-1 (C) whole cell lysates.| Product Name | TCP-1 β Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The protein TCP-1 (t complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species, TCP-1 å, ∫, ©, ∂, é, Ω, eta and theta, each encoded by a different gene. Two Ω subunits have been described: TCP-1 Ω (also designated TCP-1 Ω1) and TCP-1 Ω2. TCP-1 subunits are proposed to have independent functions in folding its in vivo substrates, the actins and tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TCP-1 &beta of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 456-535 mapping at the C-terminus of TCP-1 &beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 456-535 mapping at the C-terminus of TCP-1 β of human origin |
| Entrez Gene | 10576 |
|---|
Application
Western blot analysis of TCP-1 β expression in non-transfected: (A) and mouse TCP-1 β transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in ductus seminiferus and Leydig cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of TCP-1 β expression in mouse testis tissue extract (A) and ZR-75-1 (B), F9 (C), HeLa (D) and HOS (E) whole cell lysates.
Application
Western blot analysis of TCP-1 β expression in mouse testis tissue extract (A) and HeLa (B) and ZR-75-1 (C) whole cell lysates.| Application Notes | recommended for detection of TCP-1 &beta of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
