Application
Western blot analysis of PGK1 expression in non-transfected 293T: (A), mouse PGK1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of PGK1 expression in non-transfected: (A) and human PGK1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PGK1 expression in non-transfected 293T: (A), human PGK1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of PGK1/2 expression in non-transfected 293T: (A), human PGK1/2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of PGK1 and PGK2 expression in HeLa (A) and JAR (B) whole cell lysates.| Product Name | PGK1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Phosphoglycerate kinases 1/2 (PGK1/2, (ATP:3-phospho-D-glycerate 1-phosphotransferase, EC 2.7.2.3) are somatically expressed, glycolytic enzymes that catalyze the transfer of a phosphoryl group from the acyl phosphate of 1,3-bisphosphoglycerate to ADP, thereby forming ATP and 3-phosphoglycerate. The human PGK gene is interrupted by 10 introns and spans 23 kilobases, and is X chromosome-linked at position Xq11-Xq13, a region implicated in prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. In addition to influencing glycolysis, the PGK1 is secreted by tumor cells and contributes to proliferative angiogenic processes as a disulfide reductase. PGK1 mediated reduction of disulphide bonds in the serine proteinase plasmin initiates the release of the tumor blood vessel inhibitor angiostatin, an event that is critical for blood vessel formation or angiogenesis in tumor expansion and metastasis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PGK1 and PGK2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 119-418 mapping at the C-terminus of PGK1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 119-418 mapping at the C-terminus of PGK1 of human origin |
| Entrez Gene | 5230 |
|---|
Application
Western blot analysis of PGK1 expression in non-transfected 293T: (A), mouse PGK1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of PGK1 expression in non-transfected: (A) and human PGK1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PGK1 expression in non-transfected 293T: (A), human PGK1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of PGK1/2 expression in non-transfected 293T: (A), human PGK1/2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of PGK1 and PGK2 expression in HeLa (A) and JAR (B) whole cell lysates.| Application Notes | recommended for detection of PGK1 and PGK2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
