Application
Western blot analysis of ANAPC2 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear and cytoplasmic staining of reaction center cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of ANAPC2 expression in non-transfected 293T: (A), human ANAPC2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear staining.| Product Name | ANAPC2 Antibody (H-295) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Comprising more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdc2/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including p55 CDC, E-cadherin and MAD2. Specifically, p55 CDC and E-cadherin directly bind to APC and activate the cyclin-ubiquitination activity of APC. In contrast, MAD2 inhibits APC by forming a ternary complex with p55 CDC and APC and thus preventing APC activation. A heterodimeric complex of either Ubc4 or UbcH10 with ANAPC2 (also known as APC2) and APC11 catalyzes the ubiquitination of human securin and cyclin B1. ANAPC2 contains a C-terminal cullin homology domain that binds both APC11 and UBE2C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ANAPC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 6-300 mapping at the N-terminus of ANAPC2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 6-300 mapping at the N-terminus of ANAPC2 of human origin |
| Entrez Gene | 29882 |
|---|
Application
Western blot analysis of ANAPC2 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear and cytoplasmic staining of reaction center cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of ANAPC2 expression in non-transfected 293T: (A), human ANAPC2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse kidney frozen section showing nuclear staining.| Application Notes | recommended for detection of ANAPC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
