Application
Western blot analysis of Calponin 2 expression in non-transfected: (A) and human Calponin 2 transfected: (B) 293T whole cell lysates.| Product Name | Calponin 1/2/3 Antibody (FL-297) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin) are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Calponin 1 and 3 and, to a lesser extent, Calponin 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-297 representing full length Calponin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-297 representing full length Calponin 1 of human origin |
| Entrez Gene | 1264 |
|---|
Application
Western blot analysis of Calponin 2 expression in non-transfected: (A) and human Calponin 2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Calponin 1 and 3 and, to a lesser extent, Calponin 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
