Application
Western blot analysis of galectin-4/6 expression in T84 whole cell lysate.| Product Name | galectin-4 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One member of this family, Galectin-4, also known as Gal-4, L36 or LGALS4 maps to human chromosome 19q13.13. The Galectin-4 protein is composed of 323 amino acids and contains two homologous carbohydrate recognition domains (CRD) and all amino acids typically conserved in the galectin family. Expression of Galectin-4 correlates with the malignant potential of human hepatocellular carcinoma (HCC) and is differentially regulated depending on cell-cell contact, serum growth factors, cell growth and cell differentiation status. Galectin-4 expression is detected in epithelial cells of the colon, rectum, intestine, and in HT29 and LS174T cell lines. Galectin-4 is underexpressed in colorectal cancer and is preferentially upregulated in cells prone to peritoneal dissemination. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of galectin-4 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA may cross-react with galectin-6 of mouse origin | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 91-230 mapping within an internal region of galectin-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 91-230 mapping within an internal region of galectin-4 of human origin |
| Entrez Gene | 16855 |
|---|
Application
Western blot analysis of galectin-4/6 expression in T84 whole cell lysate.| Application Notes | recommended for detection of galectin-4 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA may cross-react with galectin-6 of mouse origin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
