Application
Western blot analysis of PEDF expression in non-transfected 293T: (A), human PEDF transfected 293T: (B) and Y79 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human PEDF transfected HEK 293T cells (B).Application
Western blot analysis of PEDF expression in non-transfected: (A) and human PEDF transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PEDF expression in non-transfected: (A) and human PEDF transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.Application
Western blot analysis of PEDF expression in A549 whole cell lysate.Application
Western blot analysis of PEDF expression in Y79 whole cell lysate.| Product Name | PEDF Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Pigment epithelium-derived growth factor (PEDF), also known as EPC-1 (early population doubling level cDNA-1), is a glycoprotein found naturally in the normal eye. PEDF has reported neuroprotective and differentiation properties and is secreted in abundance by retinal pigment epithelium cells. It belongs to the serine protease inhibitor (Serpin) superfamily and has been reported to inhibit angiogenesis and proliferation of several cell types. The “pooling” of PEDF within the interphotoreceptor matrix places this molecule in a prime physical location to affect the underlying neural retina. Additionally, PEDF induces neuronal differentiation and promotes survival of neurons of the central nervous system from degeneration caused by serum withdrawal or glutamate cytotoxicity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PEDF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 281-405 mapping near the C-terminus of PEDF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 281-405 mapping near the C-terminus of PEDF of human origin |
| Entrez Gene | 5176 |
|---|
Application
Western blot analysis of PEDF expression in non-transfected 293T: (A), human PEDF transfected 293T: (B) and Y79 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human PEDF transfected HEK 293T cells (B).
Application
Western blot analysis of PEDF expression in non-transfected: (A) and human PEDF transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PEDF expression in non-transfected: (A) and human PEDF transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.
Application
Western blot analysis of PEDF expression in A549 whole cell lysate.
Application
Western blot analysis of PEDF expression in Y79 whole cell lysate.| Application Notes | recommended for detection of PEDF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
