Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of NF2 expression in non-transfected: (A) and human NF2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of NF2 expression in non-transfected 293T: (A), human NF2 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of NF2 expression in non-transfected 293T: (A), mouse NF2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.Application
Western blot analysis of NF2 expression in MCF7 whole cell lysate.| Product Name | NF2 Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Neurofibromatosis type 2 (NF2) is a dominantly inherited disorder characterized by the occurance of bilateral vestibular schwannomas and other central nervous system tumors, including multiple meningiomas. NF2 occurs in about 1 of 40,000 live births. The NF2 gene is highly penetrant; NF2-affected individuals have a 95% chance of developing bilateral vestibular schwannomas. NF2 is distinct from NF1, which is characterized by an incidence of 1 in 4000, maps to chromosome 17 and encodes a protein designated Neurofibromin, which is a large protein with a GAP domain. Genetic linkage studies of both sporadic and familial tumors suggest that NF2 is caused by inactivation of a tumor suppressor gene that maps on chromosome 22q12 and encodes a 595 amino acid protein whose function appears to be mediated by interaction with the cytoskeleton. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of NF2 isoforms 1-10 of mouse, rat and human origin by WB, IP, IF, IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 336-595 mapping at the C-terminus of NF2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 336-595 mapping at the C-terminus of NF2 of human origin |
| Entrez Gene | 4771 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of NF2 expression in non-transfected: (A) and human NF2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of NF2 expression in non-transfected 293T: (A), human NF2 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of NF2 expression in non-transfected 293T: (A), mouse NF2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization.
Application
Western blot analysis of NF2 expression in MCF7 whole cell lysate.| Application Notes | recommended for detection of NF2 isoforms 1-10 of mouse, rat and human origin by WB, IP, IF, IHC(P)and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
