Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of COL1A1 expression in Hs 294T (A) and 143.98.2 (B) whole cell lysates.| Product Name | COL1A1 Antibody (H-197) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1021-1217 mapping within an internal region of Collagen α1 Type 1 of human origin |
| Modification Notes | The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Collagen α1 Type I of human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1021-1217 mapping within an internal region of Collagen &alpha 1 Type 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1021-1217 mapping within an internal region of Collagen α1 Type 1 of human origin |
| Entrez Gene | 1277 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of COL1A1 expression in Hs 294T (A) and 143.98.2 (B) whole cell lysates.| Application Notes | recommended for detection of Collagen α1 Type I of human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
