Application
Western blot analysis of caspase-11 p20 expression in LPS/IFNγ treated RAW 264.7 (A), C6 (B) and RAW 309 (C) whole cell lysates.| Product Name | caspase-11 p20 Antibody (M-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Caspase-11 plays a crucial role in OLG death and pathogenesis in experimental autoimmune encephalomyelitis (EAE). Caspase-11 also leads to the synthesis of the functional form of the cytokine interleukin-1beta. Caspases are a family of mammalian proteases related to the ced-3 gene of Caenorhabditis elegans. These ced-3 orthologs mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins, and propagation of other pro-apoptotic molecules. Based on their substrate specificities and DNA sequence homologies, the 14 currently identified caspases may be divided into three groups: apoptotic initiators, apoptotic executioners and inflammatory mediators. Upon activation, caspases appear to play an important role in sequelae of traumatic brain injury, spinal cord injury and cerebral iischemia. In addition, they may also play a role in mediating cell death in chronic neurodegenerative conditions such as Alzheimer’s disease, Huntington’s disease and amyotrophic lateral sclerosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of caspase-11 of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 141-195 of caspase-11 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 141-195 of caspase-11 of mouse origin |
| Entrez Gene | 12363 |
|---|
Application
Western blot analysis of caspase-11 p20 expression in LPS/IFNγ treated RAW 264.7 (A), C6 (B) and RAW 309 (C) whole cell lysates.| Application Notes | recommended for detection of caspase-11 of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
