Application
Western blot analysis of IP3R-II expression in non-transfected 293T: (A), mouse IP3R-II transfected 293T: (B) and A-10 (C) whole cell lysates.| Product Name | IP3R-I/II/III Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Inositol 1,4,5-triphosphate (IP3) functions as a second messenger for a myriad of extracellular stimuli including hormones, growth factors and neurotransmitters. Receptor tyrosine kinases indirectly increase the intracellular levels of IP3 through the activation of phospholipases such as phospholipase C (PLC), which convert phosphatidylinositol-4,5 bisphosphate into IP3 and diacylglycerol (DAG). The inositol 1,4,5- triphosphate receptor, IP3R, acts as an inositol triphosphate (IP3)-gated calcium release channel in a variety of cell types. Three IP3 receptor subtypes have been described and are designated IP3R-I, IP3R-II and IP3R-III. IP3R-I is the predominant IP3R subtype expressed in neuronal tissues and the central nervous system, but is also expressed at high levels in the liver. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IP3R-I, II and III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 2402-2701 mapping at the C-terminus of IP3R-II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 2402-2701 mapping at the C-terminus of IP3R-II of human origin |
| Entrez Gene | 3709 |
|---|
Application
Western blot analysis of IP3R-II expression in non-transfected 293T: (A), mouse IP3R-II transfected 293T: (B) and A-10 (C) whole cell lysates.| Application Notes | recommended for detection of IP3R-I, II and III of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
