Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Antibodies tested include p-Ezrin (Tyr 146)-R: (A,B) and Ezrin (H-276): (C,D).Application
Western blot analysis of Ezrin expression in non-transfected 293T: (A), human Ezrin transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of Ezrin expression in Jurkat (A), A-431 (B), HeLa (C), NIH/3T3 (D) and F9 (E) whole cell lysates.| Product Name | Ezrin Antibody (H-276) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ezrin, Moesin and Radixin belong to a family of highly homologous actin-associated proteins that are localized just beneath the plasma membrane (1). The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins (2–5). Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor (5). Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78 (6). Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types (7,8). Despite the high degree of homology, the three proteins exhibit a distinct receptor-specific pattern of phosphorylation (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ezrin and, to a lesser extent, Radixin and Moesin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 311-586 mapping at the C-terminus of Ezrin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 311-586 mapping at the C-terminus of Ezrin |
| Entrez Gene | 7430 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human seminal vesicle tissue showing cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Antibodies tested include p-Ezrin (Tyr 146)-R: (A,B) and Ezrin (H-276): (C,D).
Application
Western blot analysis of Ezrin expression in non-transfected 293T: (A), human Ezrin transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of Ezrin expression in Jurkat (A), A-431 (B), HeLa (C), NIH/3T3 (D) and F9 (E) whole cell lysates.| Application Notes | recommended for detection of Ezrin and, to a lesser extent, Radixin and Moesin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
