Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in HL-60 (A) and HuT 78 (B) whole cell lysates.| Product Name | IL-3/IL-5/GM-CSFRβ Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The human IL-3, IL-5 and GM-CSF receptors are each composed of both unique a subunits and a common ∫ subunit. The a subunits are low affinity ligand binding proteins while the ∫ subunits do not themselves bind ligand, but are required for high affinity binding by the a subunits (1–5). In contrast, the mouse IL-3 receptor has two distinct ∫ subunits, one that functions only in IL-3 mediated cell signaling and a second that is shared with IL-5 and GM-CSF (6). The murine ∫-subunits are 91% homologous at the amino acid level but only 56% homologous to the human ∫ subunit (6). Although neither the murine nor the human ∫ subunit contains tyrosine kinase domains, both activate tyrosine phosphorylation mediated signaling pathways (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IL-3/IL-5/GM-CSFR &beta chain of human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of IL-3/IL-5/GM-CSFR&beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of IL-3/IL-5/GM-CSFRβ of human origin |
| Entrez Gene | 1439 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human salivary gland tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in HL-60 (A) and HuT 78 (B) whole cell lysates.| Application Notes | recommended for detection of IL-3/IL-5/GM-CSFR &beta chain of human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
