Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in non-transfected 293T: (A), human IL-3/IL-5/GM-CSFRβ transfected 293T: (B) and HL-60 (C) whole cell lysates.| Product Name | IL-3/IL-5/GM-CSFRβ Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific å subunit and a signal transducing ß subunit common to the IL-3/IL-5/GM-CSF receptors. The carboxy terminus of the ß subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-3/IL-5/GM-CSFR &beta chain of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 598-897 mapping at the C-terminus of IL-3/IL-5/GM-CSFR&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 598-897 mapping at the C-terminus of IL-3/IL-5/GM-CSFRβ of human origin |
| Entrez Gene | 1439 |
|---|
Application
Western blot analysis of IL-3/IL-5/GM-CSFRβ expression in non-transfected 293T: (A), human IL-3/IL-5/GM-CSFRβ transfected 293T: (B) and HL-60 (C) whole cell lysates.| Application Notes | recommended for detection of IL-3/IL-5/GM-CSFR &beta chain of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
