Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Pax-8 expression in non-transfected: (A) and mouse Pax-8 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Pax-8 expression in non-transfected 293T: (A), mouse Pax-8 transfected 293T: (B) and NAMALWA (C) whole cell lysates.Application
Western blot analysis of Pax expression in NAMALWA whole cell lysate.| Product Name | Pax Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The Pax gene family of nuclear transcription factors is comprised of nine family members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax genes contain paired domains with strong homology to genes in Drosophila which are involved in programming early development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Pax-1&ndash3 and Pax-5&ndash9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 72-150 of Pax-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 72-150 of Pax-2 of human origin |
| Entrez Gene | 5076 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Pax-8 expression in non-transfected: (A) and mouse Pax-8 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Pax-8 expression in non-transfected 293T: (A), mouse Pax-8 transfected 293T: (B) and NAMALWA (C) whole cell lysates.
Application
Western blot analysis of Pax expression in NAMALWA whole cell lysate.| Application Notes | recommended for detection of Pax-1&ndash3 and Pax-5&ndash9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
