Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing membrane and cytoplasmic staining of cells in seminiferous ducts.| Product Name | MRP1 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1. MRP1 is an integral membrane protein that contains an Mdr-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood. The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter) and MRP3, which are both conjugate export pumps ex-pressed predominantly in hepatocytes. MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells. Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane. MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired. MRP6 protein is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues yet at much lower levels of expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MRP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-70 mapping at the N-terminus of MRP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-70 mapping at the N-terminus of MRP1 of human origin |
| Entrez Gene | 4363 |
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Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing membrane and cytoplasmic staining of cells in seminiferous ducts.| Application Notes | recommended for detection of MRP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
