Application
Western blot analysis of ABC-me expression in HEL 92.1.7 (A), K-562 (B), TF-1 (C), LADMAC (D) and MCP-5 (E) whole cell lysates.Application
Western blot analysis of ABC-me expression in MOLT-4 whole cell lysate.Application
Western blot analysis of Abc-me expression in CCRF-CEM (A) and MOLT-4 (B) whole cell lysates.| Product Name | ABC-me Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | ATP-binding cassette (ABC) transporters constitute a group of highly conserved cellular transmembrane proteins (1), that participate in diverse physiological processes by coupling ATP hydrolysis to the transport of a variety of substrates across cell membranes (2,3). A newly identified ABC transporter, ABC-me (for ABC-mitochondrial erythroid), localizes to the mitochondrial inner membrane and is expressed at high levels in erythroid tissues of embryos and adults (4). ABC-me is a half-ABC transporter and comprises one ATP binding domain and three transmembrane loops, which suggests that ABC-me functions as either a homo- or heterodimer (5). ABC-me, a 482 amino acid protein, is strongly induced by the transcription factor GATA-1, which is essential for normal erythoropoiesis. In addition, ABC-me contains GATA-binding sites that are normally present in promoters or enhancers of genes expressed selectively in erythroid cells. ABC-me is induced during erythroid maturation in cell lines and primary hematopoietic cells, and its overexpression enhances hemoglobin synthesis in erythroleukemia cells. ABC-me may mediate critical mitochondrial transport functions related to heme biosynthesis (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Abc-me of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 401-520 of Abc-me of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 401-520 of Abc-me of human origin |
| Entrez Gene | 23456 |
|---|
Application
Western blot analysis of ABC-me expression in HEL 92.1.7 (A), K-562 (B), TF-1 (C), LADMAC (D) and MCP-5 (E) whole cell lysates.
Application
Western blot analysis of ABC-me expression in MOLT-4 whole cell lysate.
Application
Western blot analysis of Abc-me expression in CCRF-CEM (A) and MOLT-4 (B) whole cell lysates.| Application Notes | recommended for detection of Abc-me of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
