Application
Western blot analysis of Smac expression in non-transfected 293T: (A), human Smac transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Smac expression in non-transfected 293T: (A), mouse Smac transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing membrane staining.Application
Western blot analysis of Smac expression in HeLa (A) and DU 145 (B) whole cell lysates.| Product Name | Smac Antibody (H-177) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 63-239 mapping at the C-terminus of Smac of human origin |
| Modification Notes | The activation of caspases is a key regulatory step in apoptosis (1). Once cytochrome c is released from the mitochondria into the cytosol, it binds Apaf-1 to form an oligomeric cytochrome c/Apaf-1 complex, which induces caspase activation (2,3). Inhibitors of Apoptosis Proteins (IAPs), are a family of proteins that regulate the cytochrome c/Apaf-1 caspase activating pathway (4). Like cytochrome c, Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and preventing them from inhibiting caspases (4,5). In healthy cells, Smac is a mitochondrial protein, but when cells undergo apoptosis, Smac is released into the cytosol (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Smac of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 63-239 mapping at the C-terminus of Smac of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 63-239 mapping at the C-terminus of Smac of human origin |
| Entrez Gene | 56616 |
|---|
Application
Western blot analysis of Smac expression in non-transfected 293T: (A), human Smac transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Smac expression in non-transfected 293T: (A), mouse Smac transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing membrane staining.
Application
Western blot analysis of Smac expression in HeLa (A) and DU 145 (B) whole cell lysates.| Application Notes | recommended for detection of Smac of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
