Application
Western blot analysis of GM-CSFRα expression in WEHI-3 (A) and AMJ2-C8 (B) whole cell lysates.| Product Name | GM-CSFRα Antibody (M-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-130 of GM-CSFRα of mouse origin |
| Modification Notes | The human IL-3, IL-5 and GM-CSF receptors are each composed of both unique a subunits and a common ∫ subunit. The a subunits are low affinity ligand binding proteins while the ∫ subunits do not themselves bind ligand, but are required for high affinity binding by the a subunits (1–5). In contrast, the mouse IL-3 receptor has two distinct ∫ subunits, one that functions only in IL-3 mediated cell signaling and a second that is shared with IL-5 and GM-CSF (6). The murine ∫-subunits are 91% homologous at the amino acid level but only 56% homologous to the human ∫ subunit (6). Although neither the murine nor the human ∫ subunit contains tyrosine kinase domains, both activate tyrosine phosphorylation mediated signaling pathways (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GM-CSFRα of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-130 of GM-CSFR&alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-130 of GM-CSFRα of mouse origin |
| Entrez Gene | 12982 |
|---|
Application
Western blot analysis of GM-CSFRα expression in WEHI-3 (A) and AMJ2-C8 (B) whole cell lysates.| Application Notes | recommended for detection of GM-CSFRα of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
