| Product Name | 5-LO Antibody (H-120) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 106-225 mapping near the N-terminus of 5-LO (5-lipoxygenase) of human origin |
| Modification Notes | 5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells (1). 5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis (1,2). The cellular localization of 5-LO varies between cell types (3). In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope (4). In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane (5). This intracellular shuttling of 5-LO is dependent on the association with various signaling molecules, phosphorylation and the presence of a distinct nuclear localization signal, which is encoded at the amino terminus of 5-LO (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 5-LO of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 106-225 mapping near the N-terminus of 5-LO (5-lipoxygenase) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 106-225 mapping near the N-terminus of 5-LO (5-lipoxygenase) of human origin |
| Entrez Gene | 240 |
|---|
| Application Notes | recommended for detection of 5-LO of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
