Application
Western blot analysis of SLP-76 expression in non-transfected 293T: (A), human SLP-76 transfected 293T: (B) and AML-193 (C) whole cell lysates.Application
Western blot analysis of SLP-76 expression in non-transfected 293T: (A), mouse SLP-76 transfected 293T: (B) and BJAB (C) whole cell lysates.Application
Western blot analysis of SLP-76 expression in non-transfected 293T: (A), human SLP-76 transfected 293T: (B) and AML-193 (C) whole cell lysates.Application
Immunofluor?escence staining of normal mouse heart frozen section showing cytoplasmic staining.Application
Western blot analysis of SLP-76 expression in AML 193 whole cell lysate.| Product Name | SLP-76 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 234-533 mapping at the C-terminus of SLP-76 of human origin |
| Modification Notes | The translational product of the Vav proto-oncogene is exclusively expressed in cells of hematopoietic origin and is critical for lymphocyte development and activation. However, the biochemical basis of Vav’s function is unclear. Vav contains a single SH2 domain that is required for its association with the T cell receptor (TCR). Overexpression of Vav or SLP-76 in Jurkat cells leads to NFAT activation and IL-2 production. When co-expressed, Vav and SLP-76 synergize to induce a robust basal and TCR-mediated IL-2 response. Although SLP-76 does not contain a motif that would indicate it to be a member of the tyrosine, serine/threonine or lipid kinase families, it does contain several putative SH2/SH3-binding domains and has been shown to physically associate with the adapter protein GRB2 as well as PLC g1. The discovery of SLP-76 represents an important step in elucidating the mechanism of Vav transformation and TCR-mediated NFAT activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SLP-76 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 234-533 mapping at the C-terminus of SLP-76 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 234-533 mapping at the C-terminus of SLP-76 of human origin |
| Entrez Gene | 3937 |
|---|
Application
Western blot analysis of SLP-76 expression in non-transfected 293T: (A), human SLP-76 transfected 293T: (B) and AML-193 (C) whole cell lysates.
Application
Western blot analysis of SLP-76 expression in non-transfected 293T: (A), mouse SLP-76 transfected 293T: (B) and BJAB (C) whole cell lysates.
Application
Western blot analysis of SLP-76 expression in non-transfected 293T: (A), human SLP-76 transfected 293T: (B) and AML-193 (C) whole cell lysates.
Application
Immunofluor?escence staining of normal mouse heart frozen section showing cytoplasmic staining.
Application
Western blot analysis of SLP-76 expression in AML 193 whole cell lysate.| Application Notes | recommended for detection of SLP-76 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
