Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of LAT expression in non-transfected: (A) and mouse LAT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of LAT expression in Jurkat whole cell lysate.| Product Name | LAT Antibody (FL-233) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-233 representing full length LAT of human origin |
| Modification Notes | T cell receptors activate immune responses by recognizing antigen and initiating a cascade of intracellular signal transduction events, eventually culminating in cell proliferation and differentiation. Both protein tyrosine kinases and PLC © are activated by this event. LAT, or linker for activation of T cells, is an integral membrane protein that has been shown to associate with PLC ©1, as well as GRB2 and the p85 subunit of PI 3-kinase. Binding of these signaling molecules to LAT is associated with phosphorylation of LAT by ZAP-70/Syk tyrosine kinases. LAT appears to play a role in activation of transcription mediated by AP-1 and NFAT following stimulation of the T cell receptor, suggesting that it acts as a linker protein in T cell activation. LAT protein is palmitoylated, and this modification is required for its tyrosine phosphorylation and localization to glycolipid-enriched microdomains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LAT of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-233 representing full length LAT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-233 representing full length LAT of human origin |
| Entrez Gene | 27040 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of LAT expression in non-transfected: (A) and mouse LAT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of LAT expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of LAT of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
