Application
Western blot analysis of K12 expression in non-transfected: (A) and human K12 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of K12 expression in non-transfected: (A) and human K12 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of K12 expression in non-transfected: (A) and human K12 transfected: (B) 293T whole cell lysates.| Product Name | K12 Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a C-terminal cytoplasmic domain of K12 of human origin |
| Modification Notes | K12, also known as SECTM1 (secreted and transmembrane 1), is a 248 amino acid single-pass type I membrane and secreted protein that is expressed in peripheral blood leukocytes, breast cancer cell lines, granulocytes, thymic epithelial cells and fibroblasts. Belonging to the SECTM family, K12 is a novel protein that is suggested to be involved in hematopoietic and/or immune system processes. K12 stimulates the up-regulation of IL-2Rå, ICAM-1 and CD69 on human NK cells. K12 is encoded by a gene located on human chromosome 17q25.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Malfunction or loss of p53 expression is associated with malignant cell growth and Li-Fraumeni syndrome. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of K12 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a C-terminal cytoplasmic domain of K12 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a C-terminal cytoplasmic domain of K12 of human origin |
| Entrez Gene | 6398 |
|---|
Application
Western blot analysis of K12 expression in non-transfected: (A) and human K12 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of K12 expression in non-transfected: (A) and human K12 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of K12 expression in non-transfected: (A) and human K12 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of K12 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
