Application
Western blot analysis of E-Selectin expression in non-transfected: (A) and human E-Selectin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of E-Selectin expression in U-698-M whole cell lysate.Application
Western blot analysis of E-Selectin expression in uninduced (A) and PMA-induced (B) Jurkat whole cell lysates.| Product Name | E-Selectin Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Selectins, also designated CD62 antigens, comprise a family of carbohydrate- binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. E-Selectin is expressed by cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of E-Selectin of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 311-610 mapping at the C-terminus of E-Selectin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 311-610 mapping at the C-terminus of E-Selectin of human origin |
| Entrez Gene | 6401 |
|---|
Application
Western blot analysis of E-Selectin expression in non-transfected: (A) and human E-Selectin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of E-Selectin expression in U-698-M whole cell lysate.
Application
Western blot analysis of E-Selectin expression in uninduced (A) and PMA-induced (B) Jurkat whole cell lysates.| Application Notes | recommended for detection of E-Selectin of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
