| Product Name | γENaC Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The epithelial sodium channel (ENaC) is a member of the ENaC/DEG superfamily that is located on the apical surface of cells. ENaC mediates sodium reabsorption in kidney, distal colon, lung, ducts of exocrine glands and other organs. ENaC is formed by heteromultimerization of four homologous subunits, å, ∫, © and ∂. The most frequently formed heterotetramer consists of two å, one ∫, and one © subunit, but the å subunit can be replaced by a ∂ subunit. The åENaC gene maps to human chromosome 12p13, and expresses a glycosylated protein. Both the ∫ and ©ENaC genes map to human chromosome 16p12, and the ©ENaC transcript is detected as a glycosylated protein. The carboxy-terminus of all ENaC subunits contains PY motifs, which interact with the ubiquitin protein ligase, Nedd4, to regulate intracellular sodium concentrations. Gain-of-function mutations involving the PY motif cause Liddle’s syndrome, an autosomal dominant form of hypertension, resulting from excessive renal sodium absorption. Conversely, ENaC loss-of-function mutations result in pseudohypoaldosteronism type I, a disorder characterized by salt wasting and hypotension. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of &gammaENaC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 411-520 mapping near the C-terminus of &gamma ENaC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 411-520 mapping near the C-terminus of γENaC of human origin |
| Entrez Gene | 6340 |
|---|
| Application Notes | recommended for detection of &gammaENaC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
