Application
Antibodies tested include p-C3G (Tyr 504)-R: (A,B) and C3G (H-300): (C,D).Application
Antibodies tested include p-C3G (G-2): (A,B) and C3G (H-300): (C,D).Application
Western blot analysis of C3G expression in non-transfected: (A) and human C3G transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tumor tissue showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.Application
Western blot analysis of C3G expression in HeLa whole cell lysate.| Product Name | C3G Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ras p21 is the prototype of a superfamily of GTPases that is involved in the regulation of a wide variety of cellular processes. Ras signals in its GTP-bound form but is “turned off” when bound to GDP. When unregulated or constitutively turned on by mutations, Ras signaling contributes to malignant transformation. The switch between active and inactive Ras is controlled by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). C3G was isolated in a screen for proteins that could bind the SH3 domain of the Crk proto-oncogene product. The carboxy-terminus of the C3G protein displays significant sequence similarity to Ras-GRF/Cdc25Mm and mSos and can substitute for Cdc25 function in S. cerevisiae. These observations strongly suggest that C3G is a GEF for Ras and is involved in the regulation of Ras signaling through Crk. The C3G gene maps to human chromosome 9q34.3 in proximity to the gene that encodes c-Abl, a proto-oncogene that regulates Crk. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of C3G of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of C3G of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of C3G of human origin |
| Entrez Gene | 2889 |
|---|
Application
Antibodies tested include p-C3G (Tyr 504)-R: (A,B) and C3G (H-300): (C,D).
Application
Antibodies tested include p-C3G (G-2): (A,B) and C3G (H-300): (C,D).
Application
Western blot analysis of C3G expression in non-transfected: (A) and human C3G transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tumor tissue showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Application
Western blot analysis of C3G expression in HeLa whole cell lysate.| Application Notes | recommended for detection of C3G of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
