Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Gβ 1 expression in non-transfected 293T: (A), mouse Gβ 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Gβ 1 expression in non-transfected 293: (A), human Gβ 1 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Gβ 1 expression in rat brain tissue extract.| Product Name | Gβ 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within a divergent dmain in the N-terminus of Gβ 1 of mouse origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e., a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein a, b and g polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the b and g subunits. The G protein b subunits are important regulators of G protein a subunits as well as of certain signal transduction receptors and effectors. In mammals, there are five different members of the b subunit family |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gβ 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a divergent dmain in the N-terminus of G&beta 1 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within a divergent dmain in the N-terminus of Gβ 1 of mouse origin |
| Entrez Gene | 2782 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Gβ 1 expression in non-transfected 293T: (A), mouse Gβ 1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Gβ 1 expression in non-transfected 293: (A), human Gβ 1 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Gβ 1 expression in rat brain tissue extract.| Application Notes | recommended for detection of Gβ 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
